大鼠胰十二指肠移植模型制作的手术技巧

Surgical procedures for the development of pancreaticoduodenal transplantation in rat

目的 :探索建立大鼠胰腺移植模型的手术技巧。 方法 :采用经肠道引流全胰十二指肠移植术式 ;带腹腔动脉和肠系膜上动脉的供者腹主动脉与受者腹主动脉端侧吻合 ,供者门静脉与受者左肾静脉袖套法吻合 ,供者十二指肠与受者十二指肠端侧吻合。 结果 :89例次手术中 5 1例次手术成功 ,术后移植胰腺具有内分泌功能。 结论 :这种胰腺移植模型制作切实可行 ,其成功的关键在于预防和治疗血管并发症。

Objective:To establish the model of pancreaticoduodenal transplantation in rats. Methods:The enteric drained whole pancreas duodenal transplantation model was investisated. The donor aortic segment including the celiac, superior mesenteric divergence were anastomosed with recipient abdominal aorta with end to side. The donor portal was cuff-anastomosed with left renal vein. The graft duodenum were anastomosed with the host jejunum with end to side. Results:Eighty-nine operations were performed and 51 rats were successful, of which all graft of successful case had endocrinium function. Conclusion:This model for pancreas transplantation is reliable.