H-2半相合小鼠CD34^+细胞和TK^+T细胞移植建立急性GVHD模型(英文)

Establishment of acute graft-versus-host disease models of H-2 haplotype matched CD34^+ cells with TK^+T lymphocytes transplantation

目的建立H-2半相合小鼠CD34+细胞和TK+T细胞混合移植急性GVHD模型。方法自亲代雄性代鼠骨髓分离CD34+细胞,同时取脾脏T细胞转染tk基因,两者移植雌性F1受鼠,每只输入CD34+细胞和TK+T细胞分别为1.0×105和1.0×107。单纯照射组和CD34+细胞组作对照。结果CD34++TK+T细胞组均发生急性GVHD,生存时间13.3±1.2天,受鼠死亡前外周血白细胞1.76±0.25×109/L,病理表现与单纯照射组明显不同。单纯照射组小鼠生存时间6.5±0.8天,与CD34++TK+T细胞组比较,P <0.05。CD34+细胞组和CD34++TK+T细胞组小鼠外周血检查可见Y染色体。结论H-2半相合CD34+细胞和TK+T细胞混合移植能建立急性GVHD模型。

Objective: To establish acute graft-versus-host diseases (aGVHD) models of H-2 haplotype matched CD34+ cells and TK+T lymphocytes transplantation. Methods: CD34+ cells were separated from bone marrow of male donor mice, meanwhile T lymphocytes were gained from spleens and transduced by retroviral vectors that encoded TK gene, and both were infused into female F1 recipient mice. CD34+ cells and TK+T lymphocytes each F1 recipient received was 1.0×105 and 1.0×107, respectively.Irradiated group and CD34+ group were acted as the control. Results: All recipient mice in CD34++TK+T group manifested acute GVHD symptoms and survived for 13.3±1.2 days. WBC of recipient mice were 1.76±0.25×109/L before death. Pathological manifestations of CD34+ +TK+T group were quite different from those of irradiated group. Survivals of irradiated group were 6.5±0.8 days. There was a statistically significant difference in survivals between CD34+ +TK+T group and irradiated group (P <0.05). F1 recipient mice in CD34+ cell group survived more than 30 days and had no GVHD symptoms.Chromosomes Y were detected in recipient mice in CD34+ group and CD34+ +TK+T group. Conclusions: Acute GVHD models could be established by transplantation of H-2 haplotype matched CD34+ cells and TK+T lymphocytes.