PCR-SSP HLA-B27亚型检测及意义

HLA-B27 subtyping by PCR-SSP method

目的对HLA-B27进行亚型检测并探讨其临床意义。方法对38例(AS样本24例;非AS 样本14例)血清学确定为HLA-B27阳性的样本采用PCR-SSP方法进行HLA-B27亚型分型。结果38例样本,PCR-SSP分型36例B27阳性,2例B27阴性,误差率为5.26%。36例B27阳性中,B*2704占77.78%(28/36例);24例AS样本与14例非AS样本B*2704所占比例统计学分析无显著差异(χ2=0.321,P > 0.05)。结论中国汉族人HLA-B27亚型以B*2704为主,AS与HLA-B27各亚型的特异基因可能无关。PCR方法准确、实用、快捷、可靠。

Objective: To detect the HLA-B27 subtype and investigate the possible clinical implication of genotype. Methods: Subtyping for HLA-B27 of polymerase chain reaction with sequence specific primers (PCR-SSP) method was applied. A total of 38 HLA-B27 positive samples detected by serology were entered into the study. Containing 24 samples diagnosed ankylosing spondylitis (AS), 14 diagnosed non-AS. Results: Among 38 samples detected, 36 samples were found to be HLA-B27 positive, 2 samples were negative, error rate was 5.26%. Of these 36 samples, B*2704 was 77.78% (28/36 samples). There was no significant difference between the rate of B*2704 positive sample in AS samples and non-AS samples (χ2=0.321,P >0.05) .Conclusion: The dominating subtype for HLA-B27 of Chinese is B*2704, AS is not associated with HLA-B27 subtypes, PCR-SSP has proved to be a high-sensitivity, rapid and accurate technique.