奶牛胚胎分割移植试验研究

Experimental Research on Bisection and Transfer of Dairy Cattle Embryos

用简单方法直接分割7～8日龄新鲜胚胎(一分为二),裸半胚成对移植给66头受体,90d 妊检,移植妊娠率为56.1%(37/66)。除6头流产和尚有5头待产外,已有26头受体产犊35头,其中有9对同卵双胎,双胎率为34.6%(9/26),半胚产犊率为29.2%(35/110)。对影响成对半胚移植妊娠率和半胚产犊率的因素如胚胎质量、胚胎在体外停留时间、胚胎发育阶段、黄体状况、受体牛品种等进行了较系统的研究。此外对冷冻胚胎进行了分割试验,移植妊娠率为45.5%(5/11),产3头犊牛。对快速冷冻和常规冷冻胚胎分割后的移植妊娠率进行了比较,分别为25.0%(1/4)和57.4%(4/7)。

With a simple method(i.e.without zona pelucida softening),7～8 days fresh embryos were bisected into two halves directly.Bare demi-embryos were transferred into 66 recipients followed by pregna-ncy rates of 56.1%(37/66)with 90 days pregnancy diagnosis.Besides 6 abor-tions and 5 coming parturitions,26 recipients gave birth to 35 calves,Amongthen,there were 9 identical twins with twin rates of 34.6%(9/26),andhalf embryo calving rates of 29.2%(35/120).Systematic research was madeon factors,such as embryonic quality time present in vitro,developmentstages,corpora lutea and breeds of recipients,which affected pairs of demi-embryos transfer pregnancy rates and demi-embryo calving rates.Splittingexperiment was carried on frozen embryos,followed by transfer pregnancyrates of 45.5%(5/11)and 3 calves.Bisected embryos(follwing rapid andregular freezing methods)transfer pregnancy rates(25.0% and 57.4%respectively)were compared.