非小细胞肺癌组织p53基因家族成员的不同表达及意义

Different expressions of p53 gene family members and their clinical significance in human non-small cell lung cancer

目的 探讨p5 3基因家族成员 p5 3、p63和p73在非小细胞肺癌 (NSCLC)中的不同表达及其临床意义。方法 利用免疫组化S P法在 60例NSCLC和 7例正常肺组织中检测 p5 3、p63和p73蛋白的表达。结果 在NSCLC中 ,p5 3、p63和 p73蛋白的阳性表达率分别为 61.67%( 3 7/60 )、80 .0 0 %( 4 8/60 )和 73 .3 3 %( 4 4/60 ) ;与正常肺组织相比 ,p5 3、p63和 p73蛋白阳性表达率的差异均有显著性 (P <0 .0 5 )。p5 3蛋白表达与肺癌细胞分化程度有密切关系 (P =0 .0 2 3 ) ,而与组织学类型、淋巴结转移和临床分期均无明显关系 (P >0 .0 5 )。p63蛋白表达与肺癌组织学类型 (P =0 .0 0 1)和淋巴结转移 (P =0 .0 2 8)有密切关系 ,而与细胞分化程度和临床分期无明显关系 (P >0 .0 5 )。p73蛋白表达与肺癌的临床病理特征均无明显关系 (P >0 .0 5 )。在NSCLC中 ,p63和p73蛋白表达之间呈显著正相关 (P =0 .0 0 0 1) ,p73与 p5 3蛋白表达之间无显著相关性 (P>0 .0 5 )。结论 p5 3基因家族可能与NSCLC的发生发展有关。p63和 p73蛋白有不同于p5 3蛋白的生物学功能 。

Objective To investigate the different expressions of p53 gene family members p53, p63 and p73, and their clinical significance in non small cell lung cancer (NSCLC). Methods p53, p63 and p73 protein expressions were detected in 60 NSCLC tissues and 7 normal lung tissues by immunohistochemistry. Results In NSCLC, positive rate of p53, p63 and p73 protein was 61.67%(37/60), 80.00%(48/60), 73.33% (44/60) respectively. There were significant differences in positive rate of three proteins as compared to normal lung tissue ( P <0.05). p53 protein expression was closely associated with tumor cell differentiation degree ( P =0.023), but was not associated with histological classification, lymph node metastasis and clinical stages ( P >0.05). Expression of p63 protein was closely related to lymph node metastasis ( P =0.028) and histological classification ( P =0.001), but not to cell differentiation degree and clinical stages ( P >0.05). There was no significant relationship between p73 protein expression and clinical characteristics of NSCLC ( P >0.05). A positive correlation was present between p63 and p73 protein expressions ( P =0.000 1). No statistical correlation was found between p53 and p73 ( P >0.05). Conclusion p53 gene family may be related to the oncogenesis and development of NSCLC. p63 and p73 proteins may have different biological function from p53 protein, and both might play oncogenic roles.