银杏酚酸的分离鉴定及其抗菌活性研究

Study on Isolation, Identification and the Antibacterial Activity of Ginkgolic Acids

本文以银杏外种皮为原料,研究了吸附树脂分离纯化银杏酚酸的方法和银杏酚酸的抗菌活性,并对其结构进行了鉴定。结果表明:非极性树脂D4020和弱极性树脂AB-8对银杏酚酸具有较好的吸附能力,银杏酚酸粗提液经两种树脂吸附,不同浓度乙醇梯度洗脱后,产物纯度可达90%,得率为0.91%,利用树脂精制银杏酚酸成本低、效率高,是一种有效的分离方法;通过LC/DAD/MS分析表明,银杏酚酸由五种异构体(C13:0,C15:1,C17:2,C15:0,C17:1)组成,其中C15:1含量最高,其次是C17:1;银杏酚酸主要抑制革兰氏阳性菌,对蜡状芽孢杆菌、枯草芽孢杆菌和金黄色葡萄球菌有很好的抑制作用,而对大肠杆菌等革兰氏阴性菌无抑制作用,酚酸的烷基侧链在抑菌中起了重要作用。

A method for the isolation and purification of ginkgolic acids in the bark of Ginkgo Biloba. L and the antibacterialactivity of ginkgolic acids were studied in this paper. The results showed that the non-polar D4020 resin and weak polar AB-8 resin all had excellent adsorption properties. Through both resins adsorbing with different concentration ethanol gradsdesorbing, the obtained ginkgolic acids products had 90% purity and 0.91% yield. Using the resins to purify ginkgolic acids wasan effecfive method that had lower cost and higher efficiency. Ginkgolic acids were identified to comprise five isomers (C13:0, C15:1,C17:2,C15:0, C17:1) by LC/DAD/MS analysis. The content of C15:1 was the highest and C17:1 was the next Ginkgolic acids werefound to be bactericidal against B.cereus, B.subtilis and S.aureus, but not bactericidal against E.coli.The alkyl side-chain of ginkgolicacids played an important role in antibacterial action of ginkgolic acids.