摘要
新鲜猪肺均浆离心后,经35%～55%饱和度的硫酸铵分级沉淀、Sephadex G-100凝胶柱层析、DEAESephadex A-50阴离子交换后,得到经SDS-PAGE显示为一条带的ACE纯品。200g猪肺可制备出ACE347.09U,比活力29.029U/mg,活力回收12.53%。用双倒数作图法得出ACE的酶反应动力学常数Km为3.168mmol/L,Vmax为6.165nmol/min。两个月的保藏后,试验证明所提酶活力保持稳定。
The fresh hog lung was homogenated and centrifugated with 35%～55% saturation ammonium sulfate fraction. Thenby using of Sephadex G-100 gel filtration and DEAE Sephadex A-50 anion exchange chromatogram column, pure ACE (Angio-tensin I-Converting Enzyme) showed as a single band by SDS-PAGE test and gave an apparent molecular weight about 185kD.The result was 347.09U ACE was obtained from 200g hog lung tissue with specific activity 29.029U/mg and activity recovery12.53%. Using Lineweaver-Burk method, the Michaelis-constant of hog lung ACE was 3.168mmol/L and Vmax 6.165nmol/min.After two months conserving, the test proved that the activity of the ACE was kept steady.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第9期71-74,共4页
Food Science
