摘要
本文首先对含有嗜酸乳杆菌(LA),保加利亚乳杆菌(LB),嗜热链球菌(ST)的分离样品进行分离,纯化,筛选出纯菌种,然后分别进行富硒试验。通过抗性筛选和三角瓶发酵培养获得富硒能力优良的菌株和富硒条件,试验结果表明:嗜酸乳杆菌(LA)富硒能力最强,其富硒条件初步确定为:选用番茄汁液体培养基,亚硒酸钠添加量为18μg/ml,pH6.6,37℃,培养24h,富硒能力为62.22%。
In this paper, pure bacterium species was first separated, sublimated and screened out from the muster containingLA, LB and ST. Then rich Selenium(Se) test was processed Separately. Rich Selenium(Se) belonging bacterium species and richSe conditions have been obtained separately by resistant screening and triangle flask fermentation. As a result the tests indicated:Rich Se capability of LA was the best and its rich Se conditions were confirmed: using liquid substrate of tomato, Na2SeO318μg/ml, pH6.6, 37℃, and cultivted 24h. Finally, the content of Selenium in rich selenium strains has reached 62.22%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第9期137-140,共4页
Food Science
