家蚕微卫星标记的筛选及其在遗传多样性分析中的应用

Development and Genetic Diversity Analysis of Microsatellite Markers in Silkworm, Bombyx mori L.

利用插入片段长度在 7kb以上的家蚕基因组文库 ,采用目标重复序列杂交法筛选微卫星位点 ,获得了 8个微卫星标记。用这 8个微卫星标记对 6个家蚕品种 (菁松、兰 10、C10 8、P5 0、5 4A、法 5 0B)进行多态性研究 ,共产生 2 3个等位基因 ,平均每个位点近 3个等位基因 ,杂合度范围 0～ 0 .75 0 ,平均杂合度为 0 .5 0。表明微卫星标记能反映各品种之间丰富的多态性 ,品种间的聚类结果较好地反映了家蚕品种的化性和地理分布特性。

A 7 kb insert genomic library has been constructed from the silkworm race P50. We screened 8 microsatellite markers from the library and assayed them in 4 diverse silkworm races from China, one from Europe and one from Japan. All microsatellites were polymorphic, showing 0-4 alleles per locus (mean 3). Heterozygosity ranged from 0-0.750 (mean 0.5). The result of UPGMA analysis is up to the geographical distribution of silkworm. Microsatellites appear to be powerful and suitable markers for application in silkworm genetics and breeding.