前列腺癌粒细胞-巨噬细胞集落刺激因子受体表达的研究

Expression and Significance of Granulocyte-macrophage Colony-stimulating Factor Receptors in Human Prostate Cancer

目的 :研究粒细胞 巨噬细胞集落刺激因子 (GM CSF)受体在前列腺癌组织和 4种肿瘤细胞株中的表达及其意义。 方法 :应用SP免疫组织化学方法研究 4 8例前列腺癌和 2 0例良性前列腺增生石蜡标本中GM CSF受体α亚单位表达 ;Western印迹方法检测慢性粒细胞性白血病细胞株K5 6 2 ,乳腺癌细胞株MCF 7,转移性前列腺癌细胞株PC 3M和急性早幼粒细胞性白血病细胞株HL 6 0GM CSF受体α亚单位的表达。 结果 :4 8例前列腺癌中GM CSF受体α亚单位表达阳性病例 38例 ,阳性率为 79.2 %。其中Gleason 2～ 4分者 15例 ,5～ 8分 14例 ,9～ 10分 9例 ,GM CSF受体的阳性率与前列腺癌Gleason评分相关 ;2 0例良性前列腺增生中GM CSF受体α亚单位表达 6例 ,阳性率为 30 .0 %。 4种肿瘤细胞株K5 6 2、HL 6 0、PC 3M和MCF 7均表达GM CSF受体α亚单位。 结论 :GM CSF受体表达是前列腺癌的重要特征 ,GM CSF可能参与前列腺癌的增殖过程。

Objective: To study the expression and significance of granulocyte-macrophage colony-stimulating factor (GM-CSF) receptors in human prostate cancer. Methods: SP immunohistochemical method was employed to investigate the expression of α subunit of GM-CSF receptors in 48 cases of primary prostate cancer, 20 benign prostate hyperplasia samples and four kinds of cancer cell lines K562, PC-3M, HL-60 and MCF-7. Results: The total positive percentage of GM-CSF expression in prostate cancer was 79.2%. The positive percentages in the groups with Gleason score 2～4, 5～8, and 9～10 were 75%, 82.3% and 81.2% respectively. The four kinds of cancer cell lines had prominent GM-CSF receptor α subunit expression. Conclusion: It suggests that both hyperplastic and neoplastic prostate tissues are responsive to GM-CSF and the extensive expression of GM-CSF receptors is an important characteristic of prostate cancer.