IGF-I、Ki-67和Bcl-2在脑胶质瘤中的表达及意义

The expression and significance of IGF-I,Ki-67 and Bcl-2 in human glioma.

目的 探讨胰岛素样生长因子I(IGF -I)、核增殖指标Ki- 6 7和抑制凋亡蛋白Bcl- 2在人脑胶质瘤中的表达及意义 ,以研究IGF -I与胶质瘤细胞增殖和凋亡的关系。方法 应用免疫组织化学S -P法检测 4 5例胶质瘤组织和 10例正常脑组织中IGF -I、Ki- 6 7和Bcl- 2的表达情况。结果 IGF -I在正常脑组织中不表达 ,胶质瘤中的表达阳性率为 71 1% (32 / 4 5 ) ,随病理分级 (WHO分级 )的增高而增高 ,Ⅰ～Ⅱ级与Ⅲ～Ⅳ级的表达差异有显著性 (P <0 .0 5 ) ;随核增殖指标Ki- 6 7、凋亡相关蛋白Bcl- 2表达升高而升高 (rs=0 .732 ,rs=0 .6 98,P均小于 0 .0 1)。结论 IGF -I作为神经胶质细胞的有丝分裂因子 ,其表达随脑胶质瘤恶性程度的增高而升高 ,表明IGF -I可作为反映脑胶质瘤细胞增殖能力的指标 ;它还通过调节抑制凋亡蛋白Bcl-

Objective To explore IGF-I expression in human glioma and the relationship between its expression and Ki-67,Bcl-2.Methods Expression of IGF-I,Ki-67 and Bcl-2 were assessed by immunohistochemistry staining in 45 cases of gliomas and 10 normal brain tissues.Results IGF-I was not expressed in all normal brain tissues,while its expression rate was 71.1%(32/45)in 45 cases of gliomas. We found there was a significant difference between low grade (WHO Ⅰ～Ⅱ) and high grade (Ⅲ～Ⅳ) of gliomas(P<0.05). The expression of IGF-Ⅰincreased with Ki-67 and Bcl-2 expression rising. Conclusion Acting as a mitotic factor of glial cells,IGF-I is expressed highly with the malignant level of gliomas increasing .So it suggests that IGF-I can be regarded as an marker of proliferation ability of glial cells. IGF-I also acts on restraining apoptosis by adjusting Bcl-2 expression level.