PCR方法检测小儿血尿中CMV的临床意义

Clinical significance of the detection of cytomegalovirus DNA in children's urine by PCR method

目的 分析血尿患儿与巨细胞病毒 (cytomegalovirus,CMV)感染的关系。 方法 对 397例血尿患儿行血清检测CMVIgM (酶联免疫吸附法 ) ,得阳性 188例。对阳性者按单纯性血尿 (n =10 7)和非单纯性血尿 (n =81)分组 ;另设健康对照组 93例。应用聚合酶链反应法对 3组尿液进行CMV检测 ,以 2 4 4bp条带出现橙黄色为CMVDNA阳性。结果 单纯性血尿组、非单纯性血尿组、对照组CMVDNA阳性率分别为 75 .70 % ,17.2 8% ,15 .0 5 % ,经 χ2 检验 ,非单纯性血尿患儿与单纯性血尿患儿之差异有显著性 (χ2 =6 2 .94 ,P =0 .0 0 0 ) ;非单纯性血尿患儿与健康对照者之间的差异无显著性 (χ2 =0 .16 ,P =0 .6 90 ) ;单纯性血尿患儿与健康对照者之间差异有显著性 (χ2 =12 2 .81,P =0 .0 0 0 )。结论 CMV病毒感染可能是单纯性血尿的重要病因。

Objective To evaluate the relation between cytomegalovirus (CMV) infection and hematuria in children. Methods Seral CMV IgM was detected in 397 children with hematuria by ELISA method,188 cases were positive. The positive group was subdivided into asymptomatic group (107 cases) and symptomatic group (81 cases), and 93 healthy children were as control group. The urine of three groups was detected CMV DNA by PCR method. Results The positive rate of CMV DNA in asymptomatic group, symptomatic group and control group was 75.70% , 17.28% and 15.05% respectively, there was significant difference between symptomatic group and asymptomatic group (χ 2= 62.94 , P= 0.000 ), there was no significant difference between symptomatic and control group (χ 2= 0.16 , P= 0.690 ); and there was significant difference between asymptomatic and control group (χ 2= 122.81 , P= 0.000 ). Conclusion Infection with CMV may be an important cause of asymptomatic hematuria.