氨苄西林丙磺舒胶囊的HPLC测定

Determination of Ampicillin and Probenecid Capsules by HPLC

建立了HPLC法测定氨苄西林丙磺舒胶囊的含量。采用C18色谱柱,以甲醇-0.02mol/L磷酸二氢钾溶液(用磷酸调至pH 3.0)(50∶50)为流动相,检测波长232nm,流速1ml/min。氨苄西林和丙磺舒分别在80～800mg/ml(r=0.9992)和22～220mg/ml(r=0.9996)范围内线性关系良好,平均回收率分别为99.2%和99.5%。

A HPLC method for determination of ampicillin and probenecid capsules was established. A C18 columnwas used with the mobile phase of methanol-0.02mol/L KH2PO4 solution (adjusted pH to 3.0 by phosphoric acid)(50∶50) at the detection wavelength of 232nm. The flow rate was 1ml/min.The calibration curves of ampicillin and probenecidwere linear in the concentration range of 80～800mg/ml(r=0.9992) and 22～220mg/ml(r=0.9996), respectively.The average recoveries were 99.2% and 99.5%, respectively.