肥胖大鼠血浆摄食相关肽和血脂水平变化

Plasma levels of feeding-related peptides and lipid in diet-induced obese rats

目的 :研究高能饲料对大鼠体重和摄食量的影响 ,探讨食源性肥胖的发生机制。方法 :(1)通过高能饲料喂养 ,建立食源性肥胖大鼠模型 ,分为 :①食源性肥胖 (DIO)组。②肥胖抵抗 (DR)组。③对照组。(2)每日观察体重及摄食量 ,共4周。(3)ELISA方法检测血浆MCH和α_MSH水平。结果 :(1)高能饲料喂养下的大鼠体重高于普通饲料喂养大鼠 ,并有50%大鼠发育为DIO。(2)与对照组和DR组相比 ,DIO组体重和摄食量明显增加 (P<0.001)。(3)与对照组相比 ,DIO组血浆MCH水平升高 ,α_MSH水平降低 ,并伴有高脂血症。结论 :高能食物可以导致具有肥胖倾向的大鼠体重增加 ,而DR鼠通过完善的代偿调节机制 ,维持正常体重 ;DIO鼠的血浆MCH水平升高以及α_MSH水平降低可能是导致其肥胖的外周发病机制之一 ;提示肥胖的发生即受环境因素影响 ,又与遗传因素密切相关。

Objective:To investigate the effect of high_fat diet on body weight and food intake of rats and to explore the possible mechanisms of diet_induced obesity(DIO),using enzyme-linked immunosorbent assay(ELISA).Methods:1.DIO rat model was established by feeding high_fat diet to rats for14weeks.2.Rats were randomly divided into3groups:DIO group,diet resistant group and control group;3.Food intake and body weight were measured daily for4weeks.4.The level of MCH andα_MSH in blood were measured by ELISA.Results:After high fat diet feeding for14weeks,about50%rats developed diet_induced obesity.The food intake and body weight of DIO group were increased significantly compared with DR and conˉtrol group(P<0.001).The DIO rats were higher level of MCH(P<0.01)and lower levelα_MSH(P<0.05)than control group.Conclusion:A decrease ofα_MSH level and an increase of MCH in blood may associate with obesity.