胸苷酸合成酶在大肠癌原发灶和相应淋巴结转移灶表达的差异

Differential expression of Thymidylate synthase in colorectal cancer and matestatic lymph nodes

目的 :研究是否存在大肠原发癌与相应淋巴结转移灶胸苷酸合成酶 (TS)表达水平的差异。方法 :收集石蜡包埋的大肠癌组织51例 ,淋巴结转移组织21例 ,正常大肠组织15例 ,大肠腺瘤组织8例 ,应用免疫组化方法研究了TS在正常大肠粘膜、大肠腺瘤 ,原发大肠癌和淋巴结转移癌中的表达规律。结果 :(1)正常大肠粘膜组织及腺瘤组织均不表达TS ,51例大肠癌组织中30例呈阳性表达 ,阳性率为58.8%。大肠癌组织TS表达水平与正常大肠粘膜组织及腺瘤组织相比有显著升高 (P<0.01) ;(2)淋巴结转移灶TS的表达在表达水平、染色范围和染色深度三个方面均高于原发灶 ,二者TS表达水平存在显著差异 (P<0.05)。结论 :大肠癌原发灶和淋巴结转移灶之间的TS表达水平存在显著差异,原发灶TS蛋白表达水平不能预测转移灶对以5 -FU为基础的化疗的反应 。

Objective:To explore whether there is significant difference of Thymidylate synthase(TS)exˉpression in primary and metastatic tumors and to determine that TS levels measured in primary colorectal cancer does not reflect the levels observed in the same patient's metastatic lesions and cannot be used to predict the response of metastatic cancer to chemotherapy.Methods:51colorectal carcinoma,21matestatic lymph nodes,15normal tissue and8adenoma samples obtained in operation were stained by immunohistochemical staining.TS expression in colorectal carcinoma tissues,adenoma tissues,matestatic lymph nodes and normal colorectal tisˉsues was observed.Results:An increased level of TS expression was shown in the carcinoma tissues as comˉpared with the normal mucosa tissues and adenoma;TS levels measured in the metastatic lesions are signifiˉcantly higher than the levels observed in the same patient's primary colorectal tumors(P<0.05).Conclusion:There is a significant difference between TS staining in primary and metastatic tumors(P<0.05).TS levels measured in the primary colorectal cancer do not reflect the levels observed in the same patient's metastatic lesions and cannot be used to predict the response of metastatic cancer to5-FU based chemotherapy.