胶体金免疫渗透试验定量检测D-dimer评价

Evaluation of quantitative detection of plasma D-dimer with gold colloids immunofiltration

目的 :通过与酶联免疫吸附试验 (ELISA)对比 ,对胶体金免疫渗透试验 (GIA)定量检测D -dimer进行评价。方法 :同时用酶联免疫吸附试验和胶体金免疫渗透试验测定血浆D -dimer含量 ,并对结果进行比较。结果 :取cut -off值为0.30mg/L ,ELISA法检测D -dimer的敏感性、特异性为90.7 %、94.5 % ,GIA法为92.5 %、96.2% ;对两种方法进行配对资料t检验 ,无显著性差异 (P>0.05) ;按照NCCLSEP9 -A文件对两种方法进行直线回归分析 ,结果相关性较好 (r=0.9966)。结论 :GIA试验具有ELISA的准确、定量的优点 ,同时又有其自身的快速、简便的特点 ,非常适用于临床实验室常规及急症应用。

Objective:To evaluate quantitative detection of plasma D-dimer with gold colloids immunofilˉtration(GIA)in comparison with ELISA.Methods:Use ELISA and GIA at the same time to detect plasma D-dimer,and compared the results.Results:When cut-off value was0.30mg/L,sensitivity and specificity of ELISA and GIA respectively were90.7%、94.5%and92.5%、96.2%.paired t-test of the two methods showed no significant difference(P>0.05).According to NCCLS EP9-A,the results of linear regression and correlaˉtion of GIA and ELISA showed good relationship(r=0.9966).Conclusion:GIA has not only the advantageous analytical property of ELISA with accuracy and quantitation,but also specialities of its own with simplicity and rapidity,so it fits routine and emergent use in the clinical labs very much.