β-环糊精键合固定相分离纯化银杏叶提取物和银杏黄酮

Purification of Ginkgo biloba leaf extract and flavone on β-cyclodextrin-Superose 12 pg

目的 考察以β-环糊精(β-CD)为键合固定相的色谱柱分离纯化银杏叶提取物和银杏黄酮的影响因素。方法 以色谱图中色谱峰的个数和目标物为离度为指标,采用低压液相色谱技术对银杏叶提取物和银杏黄酮进行分离纯化。同时比较固定相、流动相、缓冲液、pH值、洗脱速度和上样体积对分离结果的影响。结果 氯代环氧丙烷偶联β-CD-Superose 12 pg键合相能够较好地分离银杏叶提取物和银杏黄酮,优于葡聚糖凝胶Sephardex LH-20。结论 β-环糊精键合固定相适用于银杏叶提取物和银杏黄酮的分离与纯化。

Object Study on the effect factors of chromatographic column bonded by β-cyclodextrin(β-CD)-Superose 12 pg for separation and purification of Ginkgo biloba leaf extract (GBLE) and Ginkgo biloba flavone (GBF). Methods Nomuber of chromatographic peaks and resolution of desired peak as indexes, low pressure column chromatography was carried out to separate and purify GBLE and GBF. The separation conditions including stationary phase, mobile phase, buffer, pH value, eluotropic rate, and loading capacity were analyzed. Results Column bonded byβ-CD-Superose 12 pg has better efficiency than Sephadex LH-20 to separate and purify GBLE and GBF. Conclusion The new chromatographic stationary phase is suitable to isolation and purification of GBLE and GBF.