摘要
目的 研究不同浓度的稀土元素镧(La2+)对3种大黄根生物量和蒽醌次生代谢产物产量的影响。方法采用统计学分析方法,研究稀土元素La3+对两种掌叶大黄单克隆毛状根DH5c、DH7a和液体培养非转化根NOR生物量和蒽醌化合物合成的影响。结果 La3+浓度对大黄根生物量积累有显著影响,培养基中La3+为10 mg/L时,对大黄根生长表现出明显的抑制作用;La3+浓度对3种大黄根的蒽醌产量有极显著影响,当培养基中La3+1.0mg/L时,蒽醌产量最高;经稀土离子处理的3种大黄根中芦荟大黄素和大黄酸显著高于大黄素、大黄酚和大黄素甲醚。结论 培养基中添加适宜浓度的稀土离子La3+对掌叶大黄单克隆毛状根的生物量积累和蒽醌类化合物的合成具有明显的促进作用。
Object To study the effect of rare-earth elements La3+ on growth of hairy roots and normal roots of Rheum palmatum L and their yield of anthraquinones. Methods The biomass and an- thraquinone yield of two hairy clones DH5c, DH7a, and normal root NOR cultures of R. palmatum were evaluated statistically after various concentration of La3+ were administrated into culture medium. Results Significant differences of biomass yield and anthraquinone yield were shown among six La3+ concentrations, in which 10 mg/L showed obvious inhibitory effect for root growth and 1. 0 mg/L was the best for anthraquinone yield. In general, aloe-emodin and rhein were predominant in five anthraquinones after treated with the rare-earth element. Conclusion The growth of hairy root cultures of R. palmatum and their anthraquinone yield are greatly promoted by addition of La3+ to the medium.
出处
《中草药》
CAS
CSCD
北大核心
2004年第10期1171-1174,共4页
Chinese Traditional and Herbal Drugs
关键词
掌叶大黄
毛状根
稀土元素
葸醌类化合物
Rheum palmatum L
hairy root
rare-earth element
anthraquinones