益气活血法对实验性兔急性心肌缺血内皮细胞分泌功能的影响

Effect of Invigorating Qi and Activating Blood on the Secretion of Endothelial Cell in Rabbits with Acute Myocardial Ischemia

目的 :观察益气活血法对急性心肌缺血内皮细胞分泌功能的影响。方法 :取健康新西兰兔 4 0只 ,随机分为 5组 ,每组 8只 ;设空白对照组、模型组、活心灵低剂量组、活心灵高剂量组、西药组 ,后 4组在正常饲养的基础上连续分别以生理盐水、活心灵汤 8.5 4 g/ kg、活心灵汤 17.0 8g/ kg、消心痛 2 mg/ kg灌胃 6天 ,于末次给药后 1h,在戊巴比妥钠全身麻醉下 ,结扎4组动物冠状动脉 ,造成心肌缺血动物模型。观察血浆 ET及血清 NO参数。结果 :活心灵低、高剂量组血浆 ET参数较模型组显著下降 (t=- 2 .6 35 1、- 3.2 95 6 ,P <0 .0 5 )。结论 :活心灵汤可显著降低兔急性心肌缺血血浆 ET参数。

Objective:To observe the effect of invigorating qi and activating blood on the secretion of endothelial cell in rabbits with acute myocardial ischemia.Method:40 healthy New Zealand rabbits were randomly divided into the follow groups and given the corresponding medicine by perfusion for 6 days:control group,model group(saline),Huoxinling(活心灵)low doasge(Huoxinling decoction 8.54 g/kg)group,Huoxinling high dosage(Huoxinling decoction 17.08 g/kg)group and the western medicine(sorbitrate 2 mg/kg)group.Except for the control group,the coronary artery of rabbits in the latter four groups were ligated to make the model of acute myocardial ischemia one hour after the last perfusion.Plasma ET and serum NO were detected in each group.Result:Plasma ET parameters in the Huoxinling decoction groups were much lower than that in the model group(t=-2.6351,-3.2956,P<0.05).Conclusion:Huoxinling decoction could significantly decrease the plasma ET parameters in rabbits with acute myocardial ischemia.