改良的网织红细胞富集法

Improved method for reticulocyte enrichment

目的 探讨改良网织红细胞的分离方法。方法 在淋巴细胞分离液中添加泛影葡胺,通过细胞计数、测定总RNA量及采用半定量多聚合酶链反应方法比较添加前后从外周血中分离网织红细胞量变化。结果 添加泛影葡胺后,分离细胞总数增加,提取RNA增加,早期红细胞特异的珠蛋白α-mRNA表达量增加。结论 改良后的细胞分离液分离的网织红细胞较以前显著增加。

Objective To improve the methods of isolating reticulocytes. Methods Added a little cystografin to lymphocyte isolation liquid, then compared the quantity of reticulocytes by cell counting, RNA quantity and quantity of RT-PCR of alpha globin mRNA. Results After adding cystografin, the number of isolated cells, the quantity of total RNA and the product of alpha globin mRNA RT-PCR all increased.Conclusions The number of reticulocytes increases with the improved lymphocyte isolation liquid.