在体种植后骨髓基质干细胞(BMSCs)的定位及成骨效应研究

Location and osteogenesis of bone marrow stromal cells after implantation in vivo

目的探讨骨髓基质干细胞(BMSCs)与支架材料复合,并种植到动物体内后的定位及成骨情况。方法分离培养绿色荧光蛋白(GFP)小鼠BMSCs,体外扩增后与猪脱细胞骨基质材料复合,植入裸鼠背部作为实验组,并以单纯材料植入作为对照组,8周后进行大体观察、常规组织学检查及荧光检测。结果实验组移植物周围部成骨明显,GFP阳性细胞较多;中央部材料降解吸收,GFP阳性细胞少见。对照组成骨较少。结论BMSCs作为种子细胞参与了骨重建,同时GFP示踪是观测BMSCs的一种较好方法。

Objective To study the location and osteogenesis of the mesenchymal stem cells combined with the scaffold materials in vivo. Methods The bone marrow mesenchymal stem cells (BMSCs) from the GFP expressing mouse were isolated and expanded, then combined with the porcine acellular bone extracellular matrix, and at last implanted into nude mice. After 8 weeks, the gross observation, hematoxylin and eosin staining and expression of GFP were conducted. Results More new bone formed at the periphery of the transplants, and a lot of GFP positive cells were observed at the same sites. The number of GFP positive cells was small at the center of the transplants, where the scaffold material was degraded and absorbed. Conclusions As seeding cells, BMSCs are involved in bone formation. And tracking of GFP is helpful in evaluating the function of BMSCs.