定量RT-PCR检测结直肠癌患者外周血CK20 mRNA的表达

Significance of CK20 mRNA expression in peripheral blood of colorectal cancer patients by real-time fluorescent quantitative RT-PCR

目的 :检测结直肠癌患者外周血中细胞角蛋白 2 0 (CK2 0 ) m RNA的表达水平 ,并探讨其临床价值。方法 :采用实时荧光定量 RT- PCR法检测 5 1例经病理学确诊的结直肠癌患者和 30例健康对照者外周血 CK2 0 m RNA的表达水平。结果 :结直肠癌患者中 CK2 0 m RNA阳性率为 2 7.4 5 % ,而健康对照组阳性率为 6 .6 7% ,两者相比差异有统计学意义 (P<0 .0 2 5 )。随着临床分期的提高 ,患者外周血中 CK2 0 m RNA的表达率随之提高 ,但各分期间阳性率相比无显著统计学意义 (P>0 .0 5 )。Dukes'C期与 D期患者中 >10拷贝 / ml者高于 A期与 B期患者。结论 :检测结直肠癌患者外周血 CK2 0 m RNA的表达水平可发现早期脱落的肿瘤细胞 。

Objective: To detect the expression of cytokeratin 20(CK20) mRNA in peripheral blood of colorectal carcinoma and to discuss its clinical value. Methods: Real time fluorescent quantitative RT PCR was used to detect the CK20 mRNA expression in the peripheral blood of 51 patients with colorectal carcinoma and 30 healthy volunteers. Results: 27 45% of the patients showed CK20 mRNA expression, while it was 6 67% for the control group ( P <0 025). With the progress of Dukes' stages, the expression level of CK20 mRNA increased, but there was no statistic significance ( P >0 05). More samples in Dukes' C and D than in Dukes' A and B stages showed > 10 copies/ml. Conclusion: The detection of CK20 mRNA expression in peripheral blood of patients with colorectal carcinoma may be helpful to identify early shedding tumor cells. It is also useful to monitor the progression of the disease and observe the effect of clinical treatment.