摘要
目的 :寻找新的简捷、快速、精确、灵敏的评估血脑屏障 (BBB)损伤的指标和方法。方法 :应用脑缺血再灌注损伤模型 ,造成大鼠BBB的损伤 ,采用形态学和化学定量两种新方法观察BBB对伊文思蓝的通透性 ,应用免疫组织化学法检测脑组织中ZO - 1蛋白的表达水平的变化 ,以反映BBB的损伤程度。结果 :①脑局限性缺血再灌注损伤后脑内ZO - 1蛋白水平迅速降低 ,至再灌注后 2 4h达最低水平 ,至再灌注 7d时恢复到正常水平 ;②两种方法检测的伊文思蓝最大通透率均出现在再灌注损伤第 2 4h ,其变化趋势与脑组织中ZO - 1蛋白水平的变化趋势相一致。结论 :BBB对伊文思蓝的通透性和脑组织中ZO - 1蛋白水平的变化均可用于反映BBB损伤的程度 。
Objective:To search new markers that can quantify sensitively the degree of blood-brain barrier injury and to make better the method that quantifies the Evans Blue in cerebral tissue. [WT5HZ]Methods: Cerebral ischemia and reperfusion model that can result in damaging the blood-brain barrier (BBB) was induced in SD rat. Immunohistochemistry staining and semi-quantitation analysis were performed to detect the cerebral ZO-1. Life vital signs, the Evans Blue that permeated BBB was quantified by the two new methods. [WT5HZ] Results:① ZO-1 expression decreased quickly on the injured side of cerebrum, and it reached the lowest point at 24 h post-ischemia and reperfusion. Then it increased slowly, and went back to normal till Day 7. ② Evans Blue that permeated blood-brain barrier reached the highest at 24 h post-reperfusion with the two new methods, their change trends were same to the change of ZO-1 depression in rat brain. [WT5HZ]Conclusion:The permeability of BBB to Evans Blue and the changes of ZO-1 depression in brain are the markers of degree of BBB injury. The method, quantifying the permeability of BBB to Evans Blue by fluorescence microscope, can reflect simply and sensitively the degree of BBB injury. [WT5HZ]
出处
《西北国防医学杂志》
CAS
2004年第5期329-332,F003,共5页
Medical Journal of National Defending Forces in Northwest China
