小鼠胚泡黏附时已黏附侧与未黏附侧子宫内膜蛋白质组差异的研究

Comparative study between protein profile of mice endometrium with blastocysts adhering and without blastocysts adhering

目的 :用蛋白质组相关技术分析小鼠胚泡黏附时已粘附侧与未黏附侧子宫内膜蛋白质组的差异 ,探讨小鼠胚泡在双侧子宫黏附不同步现象发生的可能机制。方法 :用固相 pH梯度双向凝胶电泳 (2D -PAGE)分别分离妊娠五天 (D5)小鼠胚泡已黏附侧与未黏附侧子宫内膜的蛋白质组。银染显色 ,PDQuest 2DE软件分析。结果 :图像分析测得两种胶的匹配率达 82 .5 % ,在等电点pI4～ 7、分子量 14 .0～ 75 .4kD范围内分离得胚泡黏附侧小鼠子宫内膜蛋白点大约 76 0个 ,胚泡未黏附侧小鼠子宫内膜蛋白点大约 72 0个 ,其中至少 6 0个蛋白点在两种子宫内膜间有 2倍以上的量变。结论 :小鼠双侧子宫内膜蛋白质的差异表达 。

Objective:Examining the difference in the protein profiling of endometrium between the blastocyst-adhering side and the side without blastocyst adhering,in order to study the asynchrony of blastocysts adhesion between two uteri.Methods:The endometrium with and without blastocyst adhering from the mice pregnant for 5 days were analyzed by two-dimensional gel electrophoresis and imaging analysis.Results:The number of matched protein spots between two gels was up to 82.5%.760 and 720 protein spots with a molecular mass between 14.0～75.4 and isoelectric points (pI)from 4～7 were catalogued from silver-stained gels of the blastocyst-adhering endometrium and the endometrium without blastocyst adhering respectively.At least 60 proteins were up-regulated or down-regulated by over two folds between two groups.Conclusion:the different expression of protein profile may be a cause underlying the the asynchrony of blastocysts adhesion between two uteri.