摘要
目的 评价大黄素 (3 甲 1,6 ,8 三羟蒽醌 ) ,一种植物雌激素对去卵巢大鼠和经 βAP1 4 0 处理的培养的神经元的作用。 方法 应用动物行为学和原代皮层神经元培养方法对大黄素进行评价。通过Morris水迷宫评价去卵巢造成的大鼠智力行为学变化和饲喂大黄素 70d大鼠的行为学变化。培养的神经元分别用 β淀粉样蛋白1 4 0 ,β淀粉样蛋白1 4 0 + 17 β 雌二醇 ,和 β淀粉样蛋白1 4 0 +大黄素处理。用流式细胞仪检测皮层神经元和培养细胞中的凋亡细胞。 结果 去卵巢大鼠的时间逃避潜伏期及距离逃避潜伏期均长于对照大鼠和经大黄素处理大鼠。去卵巢大鼠神经元和经βAP1 4 0 处理的培养神经元的凋亡百分比及荧光强度均高于饲喂大黄素动物神经元及经大黄素处理培养神经元。结论 本研究支持这样的工作假说 :大黄素能保护去卵巢大鼠及培养的神经元抗 β淀粉样蛋白1 4 0
Objective To assess a effect of emodin (3-methyl-1,6,8-tri-hydroxyanthraquinone), a phytoestrogen on OVX rats and cultural neurons treated by βAP 1-40 . Methods The effect of emodin was assessed by animal behavior and primary cortical neurons culture. Behavior recognition of rats was estimated by Morris water maze (MWM) in OVX rats compared with rats treated with OVX+emodin, in which rats were fed with emodin for 70 d. Cultured neurons were respectively treated with βAP 1-40 , the βAP 1-40 + 17-β-estradiol, and βAP 1-40 +emodin. Apoptosis of neurons from cerebral cortex and cell culture was measured by flow cytometry. Results (1) There was longer time and distance escape latency in OVX rats than that of rats treated with emodin. (2) Percentage of apoptosis and FI of neurons in OVX rats and of neurons treated with βAP 1-40 was higher than that of rats fed with emodin and of cultural neurons treated with emodin. Conclusion Research supported the working hypothesis that emodin can protect rats from OVX rats and cultural neurons from βAP 1-40 .
基金
归国人员基金资助项目 (99 5 7)
