摘要
目的 探讨白藜芦醇 (Res)对脑胶质瘤细胞 (C6细胞株 )和正常成纤维细胞 (3T3细胞株 )体外增殖的影响 ,进而观察Res诱导C6和 3T3细胞系的凋亡作用。方法 用不同浓度的Res处理C6和 3T3细胞 ,以四甲基偶氮唑蓝 (MTT)比色法检测Res对C6和 3T3细胞的增殖作用 ,通过HE染色、扫描电子显微镜 (SEM )、原位末端标记法 (TUNEL)和流式细胞仪AnnexinⅤ荧光染色 ,观察细胞的形态学结构改变 ,并定量检测细胞凋亡。结果 Res抑制了C6细胞的生长与增殖 (P <0 .0 1) ,呈浓度依赖性反应 ;Res能明显诱导C6细胞凋亡 ,经 2 10 μmol/L、12 0 μmol/LRes处理 2 4h后及对照组的C6细胞 ,其凋亡率分别为 2 9.7%、14 .6 %及 2 .1% ;相应的 3T3细胞凋亡率分别为 4 .3%、3.5 %、2 .6 %。结论 Res能通过诱导C6细胞凋亡而抑制其生长与增殖 ,但对 3T3细胞无此作用。
Objective To explore the contribution of resveratrol(Res)to the proliferation and apoptosis of brain glioma C6 cell line as well as fibroblast 3T3 cell line in vitro.Methods Methyl thiazolyl tetrazolium (MTT) assay was used to measure its effects on the proliferation of C6 and 3T3 cells cultured with different concentrations of Res for 24 h, 48 h, 72 h and 96 h. HE staining, scanning electron microscope(SEM), TUNEL fluorescence staining and the Annexin V assay by flow cytometer(FCM) were applied to detect the apoptosis induced by Res.Results Res obviously suppressed the proliferation( P <0.01) and induced the apoptosis of C6 rather than 3T3 in concentration and time dependent manner, meanwhile, as for C6, the rate of apoptosis was 29.7% , 14.6% after having been treated with 210 μmol/L and 120 μmol/L Res for 24 h, respectively. The rate of apoptosis in control was 2.1%. In contrast, the corresponding rate for 3T3 was 4.3%, 3.5% and 2.6%. Conclusion The results of this study confirm the ability of Res to suppress the proliferation of brain glioma C6 cells with a typical apoptotic feature in vitro. Therefore, Res might be considered as a possible treatment strategy for brain gliomas.
出处
《中华神经外科疾病研究杂志》
CAS
2004年第5期401-405,共5页
Chinese Journal of Neurosurgical Disease Research
基金
第四军医大学学员课外科研基金资助项目 (0 4x - 0 1 )