水分胁迫下丛枝菌根真菌对枳实生苗生长和渗透调节物质含量的影响

Effects of Arbuscular Mycorrhizal Fungi on Plant Growth and Osmotic Adjustment Matter Content of Trifoliate Orange Seedling Under Water Stress

采用盆栽试验研究了水分胁迫下接种丛枝菌根真菌摩西球囊霉(Glomus mosseae)对枳[Poncirus trifoliat(L.)Raf.]实生苗的生长和渗透调节物质含量的影响。结果表明,在土壤含水量为20％、16％和12％条件下,接种G.mosseae能够增加植株的生长(株高、茎粗、叶面积、地上部干重、地下部干重和植株干重),促进植株根系活跃吸收面积和根际土壤有效磷的吸收,提高叶片和根系可溶性糖含量的积累,降低叶片脯氨酸含量,增强植株的水分利用效率(达20％～40％),使枳实生苗的抗旱能力得到增强。土壤含水量为20％和16％条件下接种G.mosseae对植株的效果较土壤含水量为12％条件下更显著。12％的土壤含水量严重抑制G.mosseae的侵染,说明丛枝菌根侵染程度轻,其对植物的效果也差。

The effects of arbuscular mycorrhizal fungi Glomus mosseae on plant growth and osmotic adjustment matter content of trifoliate orange [Poncirus trifoliata (L.) Raf.] seedlings under water stress were studied in potted culture. The results showed that arbuscular mycorrhizalfungi inoculation could increase plant growth,such as plant height (Fig.2B), stem diameter (Fig.2A), leaf area (Fig.2C), shoot dry weight (Fig.2D), root dry weight (Fig.2E) and plant dry weight (Fig.2F), when the water content of soil was 20%, 16% and 12%. Arbuscular mycorrhizal fungi inoculation also promoted active absorbing areas of plant root (Fig.3B) and absorption of P from plant rhizosphere (Fig.4D), enhanced the accumulated quantities of soluble sugar content in leaves (Fig.4A) and roots (Fig.4B), and reduced proline content of leaf (Fig.4C). Plant inoculated with arbuscular mycorrhiza had higher plant water use efficiency than non-mycorrhizal plants (Fig.5). Drought tolerance of trifoliate orange seedling inoculated with arbuscular mycorrhiza was enhanced. Effects of arbuscular mycorrhizal fungi inoculation on trifoliate orange seedling under 20% and 16% water content of soil were more significant than under 12% water content of soil. Arbuscular mycorrhizal fungi infection was severely restrained by 12% water content of soil (Fig. 1). Thus, effects of arbuscular mycorrhizal fungi on plant probably positively related to the arbuscular mycorrhizal inoculated percentage.