一株弗兰克氏菌的分离培养及特性研究

Isolation and Characterization of a Frankia Strain from Casuarina glauca

用根瘤匀浆法 ,从粗枝木麻黄 (Casuarinaglauca)根瘤中分离到一株内生菌FCg77。生物学特性试验表明 :该菌株的适宜分离培养基为BAP培养基 ,最佳碳源为吐温 80 ,最适氮源为牛肉膏 ,能耐 5 %的盐分 ,生理类型为AB型 ,细胞壁化学组分为III型。结合回接试验结果 ,初步判定分离菌株FCg77应属于弗兰克氏菌 (Frankia)的成员。

A strain FCg77 was isolated from root nodule of Casuarina glauca by the squashing. The biological characteristics experiments demonstrated that the optimum culture medium was BAP, and the optimum carbon and nitrogen sources were tween-80 and beef. Strain FCg77 could endure 5% salinity. The strain was divided into physiological group AB, and the cell wall chemical composition was type III strain. And combined with the results of back inoculation tests, the strain of FCg77 was preliminary identified as a member of Frankia.