中度嗜盐菌Halomonas sp.BYS-1的渗透调节

Osmoregulation of a Halophilic Bacteria Strain Halomonas sp.BYS-1

Halomonassp BYS 1是一株从活性污泥中分离的中度嗜盐菌 ,它能在 0 1～ 2 6mol LNaCl的以苯乙酸为唯一碳源的基础培养基中生长。BYS 1在不同NaCl条件下生长时 ,胞内的Na+含量基本不发生变化 ;它主要通过积累K+、谷氨酸和甜菜碱来调节胞内外的渗透压平衡。当培养基中的NaCl浓度从 0 1mol L上升到 2 0mol L时 ,其胞内的K+、谷氨酸和甜菜碱分别提高了 1 9、 2 4和 1 3 6倍。

Halomonas sp.BYS-1 was a moderately halophilic bacteria strain isolated from activated sludge,It could grow on MM with NaCl concentration from 0.1～2.6 mol/L and phenyl acetic acid as sole carbon souce. When BYS-1 grew in the media with different concentrations of NaCl, there was no obvious change in its intracellular Na+ contents , it accumulated K+, glutamic acid and betaine as osmoprotectants. Its intracellular contents of K+,glutamic acid and betaine increased by 1.9,2.4 and 13.6 times, respectively, when the concentration of NaCl increased from 0.1 mol/L to 2mol/L.