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微生物细胞表面工程研究进展 被引量:2

The Microbe Cell-surface Engineering Study Progress
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摘要 微生物细胞表面工程是近年来发展起来的 ,它利用细胞表面展示技术使外源蛋白固定化于细胞表面 ,从而生产微生物细胞表面蛋白。微生物细胞表面工程可用于细胞催化剂、细胞吸附剂、活疫苗、生物传感器的开发等。微生物细胞表面工程具有广阔的应用前景 ,但是国内对这一领域的研究尚刚起步。在介绍了细胞表面工程的基础上 ,对微生物细胞表面工程技术进展进行了综述 ,并对该技术的发展给予展望。 Microbe cell-surface engineering , which use the microbe cell surface display technology to display foreign proteins on the microbe cell surface to produce cell-surface proteins, was developed in recent years. I t can be utilizedto develop cell-catalyst, cell-adsorbent , live vaccine, biosensor and so on, and have a wide application perspective. But in our county, the microbe cell-surface engineering is studied just now. This review explain the development of the microbe cell surface engineering, overview the study and progress of microbe cell-surface engineering, and look this technology into the future.
出处 《微生物学通报》 CAS CSCD 北大核心 2004年第5期106-110,共5页 Microbiology China
基金 吉林省科技发展计划项目资助 (No . 2 0 0 2 0 61 0 )
关键词 微生物细胞表面工程 细胞表面展示技术 外源蛋白 Microbe cell surface engineering, Cell surface display, Foreign proteins
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同被引文献19

  • 1朱开玲,池振明,梁丽琨,吴龙飞.海洋哈维氏弧菌溶血素蛋白在酵母菌细胞表面上的展示及其活性的测定[J].高技术通讯,2007,17(1):73-77. 被引量:3
  • 2Fu, L. L., Li, W. F., Du, H. H., Dai, W., and Xu, Z.R. 2008. Oral vaccination with envelope protein VP28 against white spot syndrome virus in Procambarusclarkii using Bacillus subtilis as delivery vehicles. Lett. Appl. Microbiol. 46(5):581-586.
  • 3Jha, R.K., Xu, Z.R., Bai, S.J., Sun, J. Y., Li, W. F., and Shen, J. 2006. Protection of Procambarus clarkia against white spot syndrome virus using vaecine expressed in Pichia pastoris. Fish Shellfish Immunol. 22(4):295-307.
  • 4Li, X., Liu, Q., Hou, L., and Huang, J. 2010. Effect of VP28 DNA vwWhite dpot syndrome virus in Litopenaeus vannamei. Aquaculture Interna- tional,18(6) :1 035-1 044.
  • 5Vaseeharan, B., Prem Anand, T., Murugan, T., and Chen, J. C. 2006. Shrimp vaccination trials with the VP292 protein of white spot syndrome virus. Lett. Appl. Microbiol. 43(2), 137-142.
  • 6Witteveldt, J., Cifuentes, C.C., Vlak, J. M., and van Hulten, M.C. 2004. Protection of Penaeus monodon against white spot syndrome virus by oral vaccination. J. Virol. 78(4):2 057-2 061.
  • 7Yi, G., Wang, Z.,Qi, Y., Yao, L., Qian, J., and Hu, L. 2004. VP28 of white spot syndrome virus is involved in the attachment and penetration into shrimp cells. J. Biochem. Mol. Biol. 37(6):726-734.
  • 8Yue,L., Chi, Z., Wang, L., Liu, J., Madzak, C., Li, J.,and Wang, X. 2008. Construction of a new plasmid for surface display on cells of Yar rowia lipolytica. Journal of Microbiological Methods,72(2) :116-123.
  • 9刘文山,闫云君.脂肪酶表面展示技术[J].中国生物工程杂志,2007,27(9):97-102. 被引量:4
  • 10叶茂,韩双艳,林影,唐语谦,王小宁.酵母表面展示-酶联免疫吸附测定法的建立[J].生物化学与生物物理进展,2007,34(10):1113-1117. 被引量:2

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