摘要
目的探讨Wistar大鼠阴茎海绵体平滑肌细胞体外培养的方法及其生物学特性。方法采用组织培养法进行培养并行免疫组化鉴定,观察其形态及测定细胞活性。结果大鼠阴茎海绵体平滑肌细胞为梭,呈长轴平行排列,体外贴壁快,在合适的条件下能够生存并保持其稳定的生物学特性。结论大鼠阴茎海绵平滑肌细胞原培养方便可行,对研究阴茎勃起及ED机制有较大帮助。
Objective: To study the methods and biological characteristics of Wistar rat smooth muscle cells cultured in vitro. Methods: Invitro tissue culture technique, the bred cells were identified by immunohistochemical technique, morphology was observed and cell count was detected. Results: The smooth muscle cell of rat were spindle-shaped and parallel along their longitudinalaxis, the attachment was rapid and maintain steady biological characteristics in fitting condition. Conclusions: The primary culture of rat corpuscavernosum smooth muscle cells is convenient and feasible, which is helpful to study of the mechanism of penile erection and erectile dysfunction (ED).
出处
《中国现代医学杂志》
CAS
CSCD
2004年第19期63-64,67,共3页
China Journal of Modern Medicine
基金
国家自然科学基金资助项目(30000063)
关键词
平滑肌细胞
阴茎海绵体
细胞营养
ED
smooth muscle cell
corpuscavernosum
cell culture
erectile dysfunction (ED).
