外周血白细胞淋巴细胞功能相关抗原-1表达对受者肾移植前后的作用

Clinical significance of peripheral blood leukocyte fuction associated antigen-1 expression in renal allograft recipients

目的监测肾移植前后外周血白细胞淋巴细胞功能相关抗原-1(LFA-1)的表达水平,了解其变化规律,探讨其在移植排斥反应中的意义。方法应用单克隆抗体-流式细胞术荧光抗体技术监测肾移植受者外周血白细胞淋巴细胞LFA-1表达水平。结果肾移植术后LFA-1表达水平较术前显著降低,并维持在低水平,发生排斥反应时显著升高,排斥时LFA-1表达水平与术后无排斥时及排斥反应控制后LFA-1比较差异有显著性,与术前比较差异无显著性。结论LFA-1在白细胞淋巴细胞上表达与排斥反应密切相关,监测FA-1的表达水平有助于肾移植排斥反应的诊断。

Objectives: To inspect the levels of peripheral blood leukocyte and lymphocyte function associated antigen-1 (LFA-1) expression in renal allograft recipients before and after renal transplantation. Methods: Monoclonal antibody-flow cytometer fluorescence technique was used and the expression of LFA-1 in lymphocytes of 23 patients with renal transplantation was detected at 1 day before operation and 3,6,9,15 days after operation by flow cytometric immunological technique. Results: The level of LFA-1 expression after transplantation was significantly decreased and maintained at a low level, and ascended suddenly during the course of graft rejection. The level of LFA-1 expression in rejection was obviously higher than in non-rejection and after the controlling of rejection. Conclusions: LFA-1 plays an important role in graft rejection. Monitoring the expression of LFA-1 is helpful to the diagnosis of the graft rejection.