IgA肾病与肿瘤坏死因子β基因多态性的关联性研究

Study on the Relation between Human IgA Nephropathy and the Polymorphrism of TNFβ

目的 探讨江苏汉族人群肿瘤坏死因子 β( tumor necrosis factorβ,TNFβ) 1 0 69位点等位基因多态性与 Ig A肾病的关系。方法 采用聚合酶链反应—限制性片段长度多态性方法对 84名健康人和 45例Ig A肾病患者 TNFβ基因的单碱基突变多态性进行了分析。结果 Ig A肾病患者的 TNFβ* 2等位基因频率 ( 67.4% )较正常人 ( 5 5 .1 % )明显升高 ( P=0 .0 1 5 ,RR=1 .68)。结论 TNFβ* 2等位基因频率与 Ig

Objective To approach the allelic polymorphism o f tumor necrosis factor β (TNFβ) and the relation of the allelic polymorphism of TNF β wiht human IgA nephropathy in the Jiangsu Han Chinese.Metho ds The single base change polymorphism in TNF β gene were determine d among 84 normal controls and 45 IgA nephropathy patients by using polymerase c hain reaction-restriction fragment length polymorphism (PCR-RFLP).R esults The gene frequency of the TNF β*2 was higher significantly i n IgA nephropathy patients that in the normal controls (IgAN patients 67.8%,norm al controls 55.4%,P<0.05,RR=1.71).Conclusion TNF β* 2 allelic frequencies is associated with IgA nephropathy.