人鼻咽癌不同部位组织相关基因表达谱的研究

Gene expression profiles in different tissues of human nasopharyngeal carcinoma

目的筛选鼻咽癌癌变发生过程中的重要基因。方法应用显微切割和荧光标记探针基因芯片杂交技术,检测鼻咽癌、癌周组织、癌旁组织及鼻咽炎症组织的基因差异表达,扫描仪扫描荧光芯片,图象处理软件分析结果。结果在检测的3组标本中,存在大量差异表达基因,涉及基因包括信号与蛋白传递、癌基因与原癌基因、免疫相关基因、凋亡基因,以及DNA结合转录和转录因子等范围。结论鼻咽癌癌变过程中有多个多种类基因参与,说明鼻咽癌癌变过程是一个复杂、多通路的过程。

Objective To screen the genes that may play an important role in the carcinogenesis of nasopharyngeal carcinoma (NPC). Methods Microdissection and cDNA genechip hybridization techniques were used to examine the differentially expressed genes in NPC tissue, the surrounding and adjacent tissues of NPC, and the nasopharyngeal inflammation tissue. The fluorescent signals on cDNA chip were scanned and the results of hybridization analyzed by image processing software. Results Many differentially expressed genes were identified between the three samples, including many different types of genes, such as those responsible for signal and protein transmission, oncogene and tumor suppression genes, immune-associated genes, apoptosis genes and DNA binding and transcription factor genes. Conclusion The carcinogenesis of NPC involves many genes of a variety of types, suggesting its complex process.