广州地区扎幌样病毒的检出及基因型分析

Detection and sequence analysis of Sapporo-like virus in Guangzhou

目的了解广州腹泻儿童是否存在扎幌样病毒(Sapporo-like virus,SLV)感染。方法在2003年秋冬季腹泻流行期间在南方医院儿科收集临床诊断为病毒性腹泻患儿的粪便标本,并采用半套式反转录聚合酶链反应(RT-PCR)方法检测。纯化阳性标本PCR产物并测序,然后将其同GenBank中的参比毒株序列比较。结果收集的169份标本中有1份SLV(CH03354)PCR扩增阳性,检出率为0.59%,同源性分析表明该毒株属于SLV GⅠ-1群。结论广州地区存在SLV所致感染,本次实验检出毒株基因型与安徽毒株不同,说明我国不同地区可能存在不同的SLV基因型。

Objective To verify the presence of Sapporo-like virus (SLV) in children with diarrhea in Guangzhou. Methods Stool specimens were collected from children with diarrhea treated in the Department of Pediatrics of Nanfang Hospital in autumn and winter, 2003, and semi-nested reverse transcriptase-polymerase chain reaction (RT-PCR) was employed for SLV detection, the positive product of which was sequenced and analyzed in comparison with the SLV sequences documented in GenBank. Results Only one positive specimen (CH03354) was found out of the 169 stool specimens collected. Sequence analysis of the capsid gene of this strain showed that it belonged to SLV GⅠ-1 cluster. Conclusion SLV exists in Guangzhou and different regions of China may have different genotypes of this virus.