利用绿色荧光蛋白筛选酵母突变株的新方法

A new method for screening mutant yeast strains with green fluorescent protein

目的建立快速简便筛选酵母突变株的方法。方法利用同源重组技术,通过转座子上的GFP基因,从酵母基因组文库中,检测发生突变的菌株,从而筛选出酵母突变株。结果应用荧光显微镜观察SC-ura 选择平板筛选出的酵母,有绿色荧光发出的即为阳性菌落。结论利用同源重组技术,初步筛选出了所需的酵母突变株。

Objective To establish a fast and simple method for screening mutant yeast strains. Materials and Methods Homologous recombination technique was used to detect mutant yeast strains in yeast genomic library, with the green fluore- scent protein gene as the reporter gene in the transposon. Results The strains that emitted green fluorescence were isolated, indicating that the gfp gene was inserted into the yeast genome by homologous recombination. Conclusion This study established a useful method for functional genome study by homologous recombination technique, and provide an alternative for gene therapeutic drug development.