胃癌高发地区白介素-1基因多态性与胃癌的关系

Association of interleukin-1 gene polymorphism with gastric cancer in a high-risk area of China

目的研究白介素-1 (IL-1)B基因+3954、-511、-31位点和IL-1受体拮抗因子(IL-1RA)基因(IL-1RN)多态性在胃癌高发地区(陕西省)普通人群和胃癌患者中的分布,探讨IL-1基因多态性与胃癌发生的关系。方法采用PCR-限制性长度片段多态性(RFLP)分析法检测高发地区169名普通人和86例胃癌患者的IL-1基因多态性。结果胃癌患者在胃癌高发区普通人群和胃癌患者中,IL-1B3954、-31、-511T/T基因型和IL-1RN2/2基因型频率无显著差别(χ2值分别为0、0.04、0.3和0,P>0.05)。结论IL-1基因多态性可能与我国胃癌高发地区(陕西省)胃癌的发生无关。

Objective To study the difference in the frequencies of interleukin-1B (IL-1) +3954, -511, -31 and IL-1RN gene polymorphism between gastric cancer patients and normal subjects in Shaanxi Province, a high risk area of gastric cancer of China. Methods The genomic DNA was extracted from the peripheral blood of 169 normal subjects and 86 gastric cancer patients in Shaanxi Province. IL-1 polymorphisms were analyzed using PCR-restriction fragment length polymorphism (PCR- RFLP). Results T/T genotype frequencies of IL-1 in +3954, -511, -31 and IL-1RN loci were similar between gastric cancer patients and normal subjects (χ2 = 0, 0.04, 0.3 and 0, P>0.05, respectively). Conclusion IL-1 gene polymorphism may be not associated with the risk of gastric cancer in this high-risk area.