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酶法合成^(13)C标记尿苷二磷酸葡萄糖的核磁鉴定 被引量：3

Characterization of ^(13)C-Labeled Uridine Diphosphoglucose of Enzymatic Synthesis by NMR
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摘要 建立了一种经济、简便的一步酶法 ,大量合成尿苷二磷酸葡萄糖 .在酶法合成中 ,用UTP代替ATP ,并建立了UTP的再生系统 ;建立了反复补加法和酶回收法 ,使酶的利用率达到最高 .UDP [4 13 C] 葡萄糖首次用该法在 0 5g规模上合成 .总产率为 69 7% .UDP [4 13 C] 葡萄糖的核磁共振 (NMR)结果表明 ,文献报道的UDPG的1H和13 C核磁共振谱中葡萄糖 4位C的归属是错误的 ,从而纠正了文献中关于UDPG核磁共振谱归属中的部分错位 . The authors set up enzymatic method of one step to synthesize UDPG in much higher yields, which is economical and cheap. In enzymatic synthesis, UTP is used to replace ATP, and repetitive system of UTP is set up. Repetitive addition and recovery of enzyme are used to take advantage of enzyme. UDP-[4- 13 C]-glucose are first synthesized on 0.5 g scale by this method. The overall yield is 69.7%. NMR spectra of 13 C-labelled UDPG make certain the assignment of C-4 of the glucose residue and suggest that part of the results in the literatures about the assignment of UDPG are wrong.
作者 祁超 刘立岩
机构地区 中国科学技术大学生命科学学院结构生物学重点实验室 中国科学院长春应用化学研究所稀土化学与物理重点实验室 中国科学院长春应用化学研究所稀土化学与物理重点实验室
出处 《中国科学院研究生院学报》 CAS CSCD 2004年第4期488-493,共6页 Journal of the Graduate School of the Chinese Academy of Sciences
基金 国家自然科学基金 ( 2 970 10 0 5 ) 中国科学院基础局重大项目 (KJ95 1 A1 5 0 4 0 2 )资助
关键词 尿苷二磷酸葡萄糖 酶法合成 一步酶法 核磁共振 UDPG, enzymatic synthesis, enzymatic method of one step,NMR
分类号 Q555.4 [生物学—生物化学] Q532.3 [生物学—生物化学]
  • 相关文献

参考文献10

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二级参考文献13

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共引文献8

同被引文献88

  • 1祁超.尿苷二磷酸葡萄糖的合成研究进展[J].化学研究,2004,15(3):73-75. 被引量：4
  • 2祁超,刘立岩.酶法大量合成^(13)C标记尿苷二磷酸葡萄糖[J].中国科学技术大学学报,2004,34(5):624-629. 被引量：3
  • 3Butler T, Elling L. Enzymatic synthesis of nucleotide sugars[J]. GlycoconJ, 1999, 16: 147-159.
  • 4Caputto R, Leloir L F, Cardini C E, et al. Isolation of the coenzyme of the galactose phosphate-glucose phosphate transformation[J]. J Bio Chem, 1950, 184: 333-350.
  • 5Calib E, Leloir L G. The biosynthsis of trehalose phosphate[J]. J Biol Chem, 1958, 231: 259-275.
  • 6Carpita N C, Delmer D P. Concentration and metabolic turnover of UDP-glucose in developing cotton fibers[J]. J Biol Chem, 1981, 256: 308-315.
  • 7Feingold D S, Baeber G A. In methods in plant biochemistry[M]. New York: Academic Press, 1990,Vol 2,39.
  • 8Moffatt J G, Khorana H G. Nucleoside polyphosphates, VIII. New and improved synthesis of uridine diphosphate glucose and flavin adenine dinucleotide using nucleoside-5'-phosphoramides[J]. J Am Chem Soc, 1958, 80:3756-3761.
  • 9Moffatt J G. Sugar nucleotide synthesis by the phosphoromorpholidate procedure[M]. Methods enzymol, 1966, VIII:137-145.
  • 10Arlt M, Hindsgaul O. Rapid chemical synthesis of sugar nucleotides in a form suitable for enzymatic oligosaccharide synthesis[J]. J Org Chem, 1995, 60: 14-15.

引证文献3

二级引证文献4

中国科学院研究生院学报
2004年 第4期

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