转PRSV复制酶基因T_2代番木瓜植株的抗病性测定

Evaluation of PRSV resistance of T_2 transgenic papaya with replicase gene

转PRSV复制酶基因的番木瓜植株,其自交及杂交二代植株经PCR检测和Southern blot杂交的结果表明:目的基因整合在番木瓜的染色体上.人工接种PRSV的Ys、Vb、Sm株系于分子检测阳性的7～8叶期植株,转基因植株均表现高抗.在定植田间的9个月中,转基因植株无一发病,而对照则全部发病.

Results from PCR and southern-blot analysis conformed that the target gene was integrated into the genome of T_2 transgenic papaya (Carica papaya) plants with replicase gene of papaya ringspot virus (PRSV). These transgenic papaya seedlings in 7-8 leaf stages were mechanically inoculated respectively with three severe strains of PRSV in South China. They showed a high resistant to all strains of PRSV. By transplanting these seedlings to field, no any symptom appeared in these transgenic plants during 9 months. On the contrary, the control plants showed very severe symptoms.