rRNA基因间隔区序列用于亲缘关系密切的根瘤菌种群鉴定及系统发育分析

Identification and phylogenetic analysis of closely related rhizobium species by rRNA gene intergenic spacer sequence

通过rRNA基因内转录间隔区的碱基序列分析,对中华根瘤菌属Sinorhizobium、慢生根瘤菌属Bradyrhizobium和中慢生根瘤菌属Mesorhizobium种间亲缘关系十分密切的菌株进行区分.结果表明,rRNA基因间隔区序列能很好地区分种间亲缘关系十分密切的菌株.用rRNA基因间隔区序列构建的系统发育树与rRNA基因序列构建的系统发育树结果十分相似.

Phylogenetic closely related species from Sinorhizobium, Bradyrhizobium and Mesorhizobium were studied by analysis of rRNA gene intergenic spacer sequence. The results indicated that sequences of rRNA gene intergenic spacer would clarify the closely related species. The phylogenetic tree constructed by sequence of 16S-23S rRNA gene intergenetic spacer is very similar with that of 16S rRNA gene sequence.