胃液中的胆汁酸测定与分析

DETERMINATION AND ANALYSIS OF BILE ACIDS IN THE STOMACH

采用高效液相色谱(HPLC)固相酶(3α-羟基类固醇脱氢酶,3α-HSD)荧光检测法,定量测定35例胃疾病患者胃液内8种结合型胆汁酸盐的含量及pH值。发现胃部病变组(胃癌组、胃溃疡组、慢性萎缩性胃炎组)胆汁酸含量显著高于正常组;胆盐对胃粘膜的损伤受pH值影响;不同类型的胆汁酸对胃粘膜损伤效应不一;且随着胆盐浓度的增加和暴露时间的延长,损害作用增强。提示胃液中胆汁酸可能是胃癌的致癌因素之一。

With an advanced HPLC, using reverse phase column, unmobile enzymatic reactor and fluorescence detector system, the 8 conjugated bite acids and pH in gastric fluid in 39 cases of stomach disorders were essayed quantitatively. The result showed that the quantity of bile acid was much higher in carcinoma of stomach, gastric ulcer and chronic gastritis than in the normal contrast. Impairment by bile acids of the mucosa of the stomach was effected by pH. The effects varied with different bile acids acting upon the mucosa of stomach. The higher the concentration of bile acids. and the longer the exposing time of bile acids, the greater the impairment. The bile acids in the stomach may be one of the factors to induce stomach carcinoma.