摘要
分别用 1μg 头、0 1μg 头和 0 0 1μg 头浓度的保幼激素类似物methoprene(蒙五一五 )体外处理亚洲玉米螟 5龄幼虫 ,测定幼虫体壁组织、血清和血细胞溶离物中酚氧化酶的活性。结果表明 :1μg 头methoprene处理组和 0 1μg 头处理组幼虫体壁组织中酚氧化酶活性与对照组相比有显著提高 (P <0 0 1) ,血清和血细胞溶离物中酚氧化酶活性也显著上升 (P <0 0 1)。将含有 2 0 羟基蜕皮酮的人工饲料饲喂亚洲玉米螟 5龄幼虫 ,处理组幼虫体壁组织的酚氧化酶活性下降 (P <0 0 5 ) ,血清和血细胞溶离物中的酚氧化酶活性均低于对照组 (P <0 0 1)。这些结果表明methoprene可以诱导亚洲玉米螟 5龄幼虫体内酚氧化酶活性的上升 ,而 2 0 羟基蜕皮酮则抑制了酚氧化酶的活性。
The effect of juvenile hormone analogue (JHA), methoprene, and 20-hydroxyecdysone (20-E) on the activity of phenoloxidase in 5th instar larvae of the Asian corn borer, Ostrinia furnacalis was investigated. The activities of phenoloxidase in integument, hemolymph and hemocyte lysate of the treated O. furnacalis larvae were determined. The results showed that when larvae were treated with 1 μg methoprene and 0.1 μg methoprene, the activity of phenoloxidase in integument of the larvae was higher than that of the control group (P<0.01), and the activities of phenoloxidase in hemolymph and hemocyte lysate also increased significantly (P<0.01). When larvae were reared with the artificial diet supplemented with 20-hydroxyecdysone, the activity of phenoloxidase in the integument of the larvae was lower than that of the control group (P<0.05), and the activities of phenoloxidase in hemolymph and hemocyte lysate decreased significantly (P<0.01). These results suggest that methoprene stimulates the activity of phenoloxidase, while 20-hydroxyecdysone, in contrast, inhibits the activity of phenoloxidase in O. furnacalis larvae.
出处
《昆虫学报》
CAS
CSCD
北大核心
2004年第5期562-566,共5页
Acta Entomologica Sinica
基金
国家自然科学基金重点项目 ( 3 993 0 0 3 0 )
