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甲基对硫磷长期污染对土壤微生物的生态效应 被引量:8

Ecological effects of long-term methylparathion contamination on soil microflora
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摘要 研究了有机磷农药甲基对硫磷长期污染对土壤微生物的影响,结果表明:污染土壤中细菌、放线菌、真菌数量变化不大,自生固氮菌和反硝化细菌数量减少,氨化细菌、亚硝化细菌、硝化细菌数量有所增加;与对照土壤相比,污染土壤呼吸作用下降29.93%,而氨化作用和硝化作用则增强。1g对照土壤(以干土计)中微生物总DNA提取量为(13.01±1.91)μg,污染土壤中则为(12.23±1.36)μg。 Long-term effects of methylparathion contamination on soil microflora were investigated.Little difference in total counts of bacteria,actinomycetes and fungi was observed between the contaminated and the non-contaminated soil.Compared with the control,the contaminated soil was slightly lower in total count of azotobacters and denitrifying bacteria and significantly higher in those of ammonifying and ammonia-oxidizing and nitrifying bacteria.The respiratory intensity of the contaminated soil decreased by 29.93% while ammonification and nitrification increased significantly over that of control soil.The total DNA extracted from the contaminated soil and the control was (12.23±1.36)μg·g^(-1) and (13.01±(1.91))μg·g^(-1) dry soil,respectively.
出处 《农村生态环境》 CSSCI CSCD 北大核心 2004年第4期48-50,共3页 Rural Eco-Environment
基金 "十五"国家科技攻关计划项目(2002BA516A01)
关键词 甲基对硫磷 长期污染 土壤微生物 生态效应 methylparathion long-term contamination soil microorganism ecological effect
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参考文献8

  • 1蔡道基 江希流 等.化学农药对生态环境安全评价研究[J].农村生态环境,1986,(2):9-13.
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  • 5张瑞福,曹慧,崔中利,李顺鹏,樊奔.土壤微生物总DNA的提取和纯化[J].微生物学报,2003,43(2):276-282. 被引量:142
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二级参考文献3

  • 1J萨姆布鲁克 EF弗里齐 T曼尼阿蒂斯.分子克隆实验指南:第二版[M].北京:科学出版社,1998..
  • 2蔡道基,江希流,蔡玉祺.化学农药对生态环境安全评价研究——Ⅰ.化学农药对土壤微生物的影响与评价[J]农村生态环境,1986(02).
  • 3Грищина Л.А.,徐道兰.农药对土壤呼吸强度的影响[J]农业环境与发展,1986(01).

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