永平铜矿浸矿细菌驯化培养研究

Study on the Domestication of Leaching-ore Bacteria from Yongping Copper Ore

以永平铜矿为研究对象,通过逐步模拟矿石环境,对浸矿细菌进行了矿石培养基与人工培养基配比的转代驯化培养研究。研究结果表明:S2-2+9K和S2-2+9K+S两个样品的细菌经过4次驯化后,溶液中Fe2+的转化速率明显提高,Fe的沉淀率明显减少,而pH逐渐下降;从调酸次数、幅度及pH下降的程度上分析,含矿石的9K+S培养基更有利于驯化硫杆菌;而从Fe2+的转化速率及Fe的沉淀率上分析,含矿石的9K培养基更有利于驯化铁杆菌;在细菌逐步驯化过程中,必须控制好合适的酸度条件,否则会造成大量Fe的沉淀。

Regarding Yongping copper ore as an object of study, the researches of domestication of leaching-ore bacteria have been conducted through gradually simulating the ore environment in some proportion of ore culture to artificial culture in this paper. The results show that after the bacteria of S_(2-2)+9K and S_(2-2)+9K+S two samples are domesticated four times, the inversion rate of Fe^(2+) in solution obviously improves, the precipitation rate of Fe obviously reduces, and while the pH value gradually descends. According to the analysis of adjusting the sour times, range and the degree of pH value descent, the ore-bearing 9K+S cultures more benefit to domesticate the Ferrobacillus. While according to the analysis of the inversion rate of Fe^(2+) and the precipitation rate of Fe in solution, the ore-bearing 9K cultures more benefit to domesticate the Thiobacillus. The acidity must be controlled well, and otherwise would result in the precipitation of large quantity Fe in the course of gradually domestication bacteria.