漆酶催化对苯基苯酚的聚合

THE POLYMERIZATION OF 4-PHENYLPHENOL CATALYZED BY LACCASE

There has been much interest in polymerization catalyzed by enzymes (“enzymatic polymerization”) as a new methodology of polymer syntheses.Recently,enzymatic synthesis of polyaromatics has been developed.Peroxidase-catalyzed polymerization of phenol and its various derivatives proceeded to give polymers with a complicated structure,which is hard to synthesize by conventional oxidative polymerizations.As a p-diphenol oxidase,laccase could be used to catalyze the oxidative reaction and macromolecule synthesis.The polymerization of 4-phenylphenol was carried out in the mixture of buffer (pH 4) and organic (15∶85,vol%) catalyzed by a laccase,produced from white rot fungus,at 35℃ for 48 h to form a polymer,2,6-poly (4-phenylphenol) with molecular weight above 80,000,which was determined by GPC.The polymer was analyzed by DSC to show that the glass transition temperature is 168.84℃ and the decomposition of this polymer took place at 301℃.The structure of this polymer is characterized by IR,H-NMR and 13 C-NMR spectra.It’s reasonable to conclude that polyphenol with phenol hydroxy groups on the molecular chains are formed through laccase enzymatic polymerization.

There has been much interest in polymerization catalyzed by enzymes ('enzymatic polymerization') as a new methodology of polymer syntheses. Recently, enzymatic synthesis of polyaromatics has been developed. Peroxidase-catalyzed polymerization of phenol and its various derivatives proceeded to give polymers with a complicated structure, which is hard to synthesize by conventional oxidative polymerizations. As a p-diphenol oxidase, laccase could be used to catalyze the oxidative reaction and macromolecule synthesis. The polymerization of 4-phenylphenol was carried out in the mixture of buffer ( pH 4) and organic ( 15: 85, vol%) catalyzed by a laccase, produced from white rot fungus, at 35 degreesC for 48 h to form a polymer, 2, 6-poly (4-phenylphenol) with molecular weight above 80,000, which was determined by GPC. The polymer was analyzed by DSC to show that the glass transition temperature is 168. 84 degreesC and the decomposition of this polymer took place at 301 degreesC. The structure of this polymer is characterized by IR, H-NMR and C-13-NMR spectra. It's reasonable to conclude that polyphenol with phenol hydroxy groups on the molecular chains are formed through laccase enzymatic polymerization.