大鼠肾皮质细胞浆脂质过氧化与庆大霉素肾毒性关系的研究

Relation between Lipid Peroxidation(LPO)in Renal Cortical Cytoplasm(RCC)And Gentamycin Nephrotoxicity

通过测定大鼠肾皮质细胞浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶活性(G-Px)及丙二醛(MDA)含量,观察肾皮质超微结构变化。发现庆大霉素可抑制肾皮质细胞浆SOD、G-Px活性,使MDA含量升高,同时出现形态学及肾功能损伤。人参总皂甙可保护SOD、G-Px活性,降低MDA含量,同时预防形态学及肾功能损害。实验表明,庆大霉素可通过抑制胞浆SOD活性、G-Px活性,使胞浆脂质过氧化程度增加,后者通过细胞毒作用导致肾功能损害。

Superoxide dismutase (SOD) activity, glutathione peroxidase (G-Px) activity, andmalondialdehyde (MDA)level in RCC in rats were measured,and the renal cortical ultra-structure wasobserved. The results showed that gentamycin could inhibit SOD and G-Px activities,and increase theMDA levels in RCC. There were morphologic changes and renal dysfunction simultaneously.Ginsenosides was able to protect the SOD and G-Px activities, and decrease the MDA levels. At thesame time, the morphologic changes and the impairment of renal function were prevented. The datasuggested that gentamycin might increase LPO levels in RCC because of the inhibition of SOD and G-Px activities in RCC,and give rise to renal dysfunction by cytotoxic reaction.