二甲基亚砜对人类食管癌细胞株作用机理的研究

Studies on the effect of dimethylsulfoxide on human esophageal cancer cell line(Eca-109)

探讨了2%二甲基亚砜(DMSO)对Eca-109细胞株作用的机理。实验结果表明:二甲基亚砜抑制了Eca-109细胞的增殖,使Eca-109细胞~3H-TdR掺入减少,对照组和实验组~3H-TdR掺入数分别为5250.3±87.2、2990.3±17.27,导致Eca-109细胞集落形成能力下降,对照组和实验组集落数分别为34.56±12.13、11.88±4.80。流式细胞术显示:对照组Eca-109细胞G0/G1,S和G2+M各期的百分比为:42.67±7.57,55.00±7.21,2.30±1.15;实验组细胞各期的百分比为71.00±9.85,20.67±15.04,8.00±5.29;(P<0.01)。实验结果提示:二甲基亚观能阻止Eca-109细胞从G0/G1期进入S期,从而可抑制细胞的生长。

The application of dimethysulfoxide (DMSO) at the concentration of 2% (Vol/Vol) to Eca-109cells resulted in the inhibition of Eca-109 cell proliferation. The cpm of incorporation of ~3H-TdR intocells in the DMSO group and the control group was 5250. 3±87. 2 and 2990. 3±17. 27 respectively.The treatment of Eca-109 cells with 2% DMSO for two weeks resulted in the reduction of the cell'sability to form colonies. The number of colony formation in the DMSO group and the control groupwas 2990. 3±17. 27 and 5250. 3±87. 2 respectively. Flow cytometry indicated that the percentagesof G 0/G 1, Sand G 2 + Mphase cells treated with and without DMSO werw 42. 67±7. 5 7, 55. 00±7. 21, 2. 30±1. 15 and in the DMSO group in different phases 71. 00±9. 85, 20. 67±15. 04, 8. 00±5. 29 respectively (P<0. 01). Our studies suggested that DMSO could block G0/G1 phase cellsentering S phase and suppressed Eca-109 cell growth.