2.5Gy中子辐射对小鼠心肌细胞损伤的实验研究

An experimental study on myocardial cell injury caused by 2.5Gy neutron irradiation in mice

〔目的〕利用 2 .5Gy 90 %的中子射线照射BALB/C小鼠 ,研究小鼠血清中反映心肌细胞损伤程度的天门冬氨酸氨基转移酶 (AST)、乳酸脱氢酶 (LDH)和肌酸激酶 (CK)、丙氨酸氨基转移酶 (ALT)活力的变化情况 ,以确定 2 .5Gy中子辐射对小鼠心肌细胞损伤的情况。〔方法〕采用二级BALB/C雄性小鼠作为实验动物 ,共计 78只。实验期间在军事医学科学院实验动物中心统一饲养。将上述小鼠分为 2组 ,包括 1个对照组和 1个实验组。采用北京清华大学的中子源分别对各动物组进行 90 %中子照射 ,照射剂量为 2 .5Gy(66只 ) ,对照组 12只。分别在照射动物之后的 6h、12h、1d、3d、5d、7d、10d、14d、2 1d、2 8d和 41d采取静脉血 ,进行AST、LDH、CK和ALT的活性测定。采用MicrocalOrigin软件包进行数理统计分析。〔结果〕2 .5Gy中子照射 5d后血清AST、LDH、CK和ALT活力均有明显升高 ,直到于照射后 2 1d～ 2 8d后仍然未恢复至正常状态 ,均显著高于正常对照组 (P <0 .0 5 )。〔结论〕2 .5Gy中子辐射可以引起心肌细胞损伤 ,进而引起血清AST、LDH、CK和ALT活力不同程度的升高。

〔Objective〕 To investigate the effects of 2.5 Gy neutron irradiation on activities of AST,LDH,CK and ALT in the mice to identify potential risks of radiation-induced myocardial cell injury.〔Methods〕BALB/C mice were irradiated by the 90% neutron at the dosage of 2.5 Gy.The activities of AST,LDH,CK and ALT in the serum were determined in 6h,12h,1d,3d,5d,7d,10d,14d,21d,28d and 41d after the irradiation.Meanwhile,the concentrations of the enzymes were determined by enzymatic methods.〔Results〕During the 5th to 41st d after the irradiation,the activities of AST and LDH in the serum were significantly higher in the irradiated mice than in the controls.The activity of CK in the serum was markedly increased in the irradiated mice as compared with the controls during the 5th to 41st d after the irradiation.The serum activity of ALT was remarkably elevated in the irradiated mice as compared with the normal controls on the 5th,7th and 21st d after the irradiation.〔Conclusions〕 Myocardial cells are injured by the 2.5 Gy neutron irradiation.