乙型流行性感冒病毒的基因快速检测

Rapid detection of influenza B viral genomes

〔目的〕建立乙型流行性感冒病毒的核酸快速诊断技术。〔方法〕采用RT -PCR方法对乙型流感病毒的HA基因进行检测 ,我们对乙型流感病毒的HA基因保守区域设计相关引物 ,进行扩增 ,并作了特异性与灵敏度比较。〔结果〕该引物只能扩增乙型流感病毒的特异性片段 ,而对其它型别的流感病毒以及麻疹、风疹、腮腺炎病毒无交叉反应。检测的灵敏度一次PCR可达 1TCID50 ,二次PCR反应可达 0 .1TCID50 。〔结论〕采用该方法从临床患者含漱液标本中的检出率 ,比采用MDCK细胞分离的阳性率更高 ,可达到迅速、正确地诊断乙型流感的实用目的。

〔Objective〕 To develop a method for raid detection of influenza B.〔Methods〕 The HA gene of human influenza B virus was detected by RT-PCR using 1.5 pairs of primers.The sensitivity and specificity of the RT-PCR method was compared to conventional viral isolation using MDCK cells.〔Results〕 The detecting rate by PCR method was much higher than that by conventional method.The dilution experiments showed that influenza B virus could be detected as few as 1TCID50 by the first PCR method and as few as 0.1TCID 50 by the second PCR method.〔Conclusions〕 PCR is a rapid,sensitive and reliable method for diagnosis of influenza B.