自身免疫性肝病患者红细胞CR1活性的变化及意义

Changes and significance of activity of complement receptor 1 expressed on red blood cells in patients with autoimmune liver disease

〔目的〕研究自身免疫性肝病患者红细胞CR1活性的变化 ,探讨其临床意义。〔方法〕红细胞天然免疫粘附功能测定 :采用红细胞在自身血浆中天然免疫粘附肿瘤细胞的方法 :5 0 μl 1.2 5 %的红细胞悬液在 10 0 μl自身血浆中与10 0 μl的 1× 10 6/ml肿瘤细胞悬液反应 ,1个肿瘤细胞粘附有 5个或以上的红细胞为一个粘附单位 ,计算粘附率。自身抗体采用酶免及免疫组化法测定。〔结果〕对 2 7例自身免疫性肝病及 3 0例正常人群研究表明 ,自身免疫性肝病患者红细胞CR1活性显著低于于正常对照人群 (P <0 .0 1) ,并且自身免疫性肝病病人红细胞CR1活性升高与自身抗体的升高密切相关。〔结论〕红细胞CR1活性的变化与自身免疫性肝病患者病情严重程度密切相关 。

〔Objective〕 To study the changes and significance of the activity of complement receptor 1 expressed on red blood cells in patients with autoimmune liver disease.〔Methods〕 A total of 50μl of red blood cells (1.25%) prepared in 0.5 M saline were mixed with 100 μl of tumor cells in 1×10 6/ml at 37℃ for 30 min.The tumor cells attached with 5 red blood cell for 2 lymphocytes andgranulocytes was counted as one rosette.The autoantibodies were assayed with ELISA and immunohistochemistry in 27 cases of autoimmune liver disease.〔Results〕 The activity of complement receptor 1 expressed on red cells was significantly lower in the patients than in healthy individuals ( P <0.05).The decreasing degree of CR1 activity in autoimmune liver disease had a good relation to the increasing level of autoantibodies.〔Conclusions〕 There is a good relationship between the activity of red cell CR1 and the severity of autoimmune liver disease.